FastQCFastQC Report
Mon 18 Jul 2016
PSAE004-NC4_S23_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePSAE004-NC4_S23_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1550069
Sequences flagged as poor quality0
Sequence length32-241
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCCC2300.00138532424.4838719
GATCGCG2400.001774841923.463717
TAGACTG7800.023.102735
CTGTGCG3002.4494494E-422.5251629
CTCTAGG4204.4997378E-621.4525361
CTAGGAG3456.292672E-419.5870953
GTATAAG18100.018.045021
AAGACCG7507.1704562E-918.0201285
TGCGTCC4401.558048E-417.9177428
GTATAGA21400.017.367531
ACAGTGC8551.9517756E-917.1243928
GTATATA34600.016.2754061
TACACAG12405.456968E-1215.4406355
ACACCGT10251.2969394E-915.38303856
GTATAGC13200.015.3580661
TACACCG11854.0017767E-1115.2068615
CTACACT10401.5934347E-915.1611674
GTATAGT18100.014.9338091
GTACGGA4600.00431330814.6903241
ACTGTAC16250.014.554728