FastQCFastQC Report
Mon 18 Jul 2016
PSAE004-NC4_S23_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePSAE004-NC4_S23_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1550069
Sequences flagged as poor quality0
Sequence length32-241
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGGGG1058.906451E-442.8940969
TATACTG25250.020.958655
GTAGGCT4159.9707184E-518.9922664
TAGGCCT3000.0064791818.7661674
TACACTG10900.018.5945
ACTGGGC4851.5635409E-518.5727028
TTATACT34250.017.0950184
GTATAAG18500.017.0417081
TAGACAG7951.4786565E-816.9957755
CTACTAG7501.4097532E-716.5142271
CTTATAC18300.015.9973883
TAGCCCC4250.002550995715.8960484
TAGGACC9255.5606506E-915.8244434
TCTATAC20800.015.698623
GTCCAGG5104.813859E-415.4544911
CTGGGCA5908.391234E-515.2673899
TATATAC34800.015.2070673
TATAGGA17850.015.1390932
TATAGTG22500.015.0129345
TAGACCG5356.922111E-414.7323195