FastQCFastQC Report
Mon 18 Jul 2016
MG319544_S10_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG319544_S10_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences692834
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATG8050.031.3962521
GGGCGAG1400.004499078324.6175167
GTATATA7600.024.1856271
ACTGTCC5153.986497E-822.3071338
CTATAGA4653.6797792E-722.2351741
ATTACAC6407.767085E-1021.5403253
GTCCTGC4906.091086E-721.1007251
GTCTATG6201.2643795E-820.3822441
TGCACCG5651.06167136E-720.3330485
GAGCCTG2850.004286255720.154696
ATAGGTC4608.317105E-619.9794313
GTACACA4209.359351E-519.1469551
TAGTGTG3000.005766342419.1469555
CTTAGAG4209.359351E-519.1469552
TATAGAG6052.1825326E-718.9887165
TATATAC8056.693881E-1018.552333
GCACAGT3100.006968186718.5293126
TATACCG6202.8234717E-718.5293125
GTGCTAG4351.2250365E-418.4867151
TAGTACT4401.3371637E-418.276644