FastQCFastQC Report
Mon 18 Jul 2016
MG319544_S10_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG319544_S10_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences692834
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAGGG1355.4114338E-542.550822
GGGCCGC1200.001555005138.295749
CCCTAGA2455.4040036E-528.1356451
GTACCCT2209.514654E-426.1107334
GTATAGT4908.3309715E-1025.7910081
TAGAGTG3151.0155714E-525.5304935
GTATAGG5402.566594E-923.402951
ACCGTCC2951.9091135E-423.3668928
TATAGTG6903.6379788E-1223.310455
GTCCTAG3102.6709697E-422.2362371
CTAGCAC4653.6780875E-722.2362373
CCTTACA4855.516504E-721.3192772
ACCGTGC3253.6760472E-421.2099468
TAGTATG4906.088303E-721.1017344
AGACTGT6059.5315045E-920.8885866
AGAGTGT3304.075198E-420.8885846
CATATAC9400.020.7774753
TATACAG8950.020.5384965
AGGACCG2800.003867666720.5155755
GTATATA6751.5097612E-920.4243951