FastQCFastQC Report
Mon 18 Jul 2016
MG319031_S8_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG319031_S8_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences781578
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGG1509.899049E-538.407739
CGCGCGG1651.7342725E-434.9161158
TATACTG8450.027.2717575
CCGTGCG2250.001066574525.6051529
CTTAGGC4059.420182E-825.605153
GGACTGC4601.1654265E-825.0485176
AGCGCGC2300.001212422725.0485176
CCTAGAC3901.9380423E-623.6355233
TAGACTC2650.00276377821.7402235
TACACCG8500.021.689075
GTATAGG5856.2591425E-921.6658971
GCCTGTG2700.003080367321.3376267
GTCCTGG3303.9962924E-420.9496691
GTGTAAG6251.3407771E-820.279281
TAGACCG4608.108696E-620.0388135
AAGCGCG4056.919745E-519.9151175
GTCCTAT4658.902631E-619.8233431
ACTCCGC2950.005144159319.529358
TACACTG7152.9795046E-919.3381565
GTCTATA6001.9393337E-719.2038651