FastQCFastQC Report
Mon 18 Jul 2016
MG319031_S8_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG319031_S8_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences781578
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTGGG1900.002353574135.1941235
CCCTGAG2005.355574E-428.8093852
GTCTATG5654.1836756E-922.4356291
GTCTAGC4153.321211E-622.2144661
AGACCGG2600.002472645122.1610646
CCAGGAC3152.9160356E-421.9500083
AGCGTGC3203.2435977E-421.6070388
GTACCCT2700.003078141721.3402865
GTCCTAG3303.9929323E-420.9522781
CCCTTAA3303.9929323E-420.9522781
GAGCGTG3354.4194324E-420.639567
TCTAGAC5108.686511E-720.3360373
GTATAGA6901.9117579E-920.0413131
GCCTATG5851.4835314E-719.6987251
CCCTAAG3556.532616E-419.4767672
CTTATAC7804.2200554E-1019.2062573
TATACTG10850.019.1177485
GTATTAT14100.018.7976131
GTCTTAG6152.511224E-718.7378141
ATAGTGG4951.5250738E-518.6242498