FastQCFastQC Report
Mon 18 Jul 2016
MG318865_S7_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG318865_S7_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences879573
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGC2253.00371E-530.6665363
GTCCTAG4103.4924597E-928.0486641
GTCTCGG1750.00981169826.2856031
CCCAGAG3201.139294E-525.1561432
CTTAGGG3401.8221253E-523.6763733
ACAGTGC4902.3286702E-823.469298
CCCTAAG3002.1258635E-422.9999031
GTATACT9650.022.6423914
ACAGGGC3052.3776063E-422.6228568
TATACTA7800.022.1152925
TATACTG8900.021.9662025
GTCTAGG4203.752395E-621.9046691
CCTCAGT3152.9574987E-421.9046691
ACACAGT5856.4046617E-921.623846
GTATAGG5406.516893E-821.2962071
GTACTAC3253.652951E-421.230681
TGCACTG6008.574716E-921.0832445
GTATAGA7651.4551915E-1121.0456621
TAGACTG4956.653827E-720.9090045
GTATGGG6601.1277734E-920.9090021