FastQCFastQC Report
Mon 18 Jul 2016
MG318865_S7_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG318865_S7_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences879573
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG7300.028.3536785
TATACCG6202.0008883E-1124.1107885
GCAGGGC2901.6904784E-423.7910163
ACCGTCC2901.6904784E-423.7910168
CTAGGAC4002.4586225E-622.9979823
GTCTAGG4552.959132E-722.7452561
TAGCCTG3052.3789512E-422.6209665
CGCAGTG3102.655663E-422.2561137
ACCGCTC4153.3845845E-622.1667298
TAGGACT5755.2550604E-921.998074
GTCTAGA4355.0898398E-621.147571
CTATACC5457.190101E-821.0990664
GTCCTGG3455.468392E-419.9982451
GTACTAG6351.6478225E-819.9195121
CTACACT6401.8033461E-819.763894
CTCTTAG4158.4788866E-519.3958871
ACAGACC4751.08894E-519.366728
ACACTGT7203.3342076E-919.1649846
GTATAGG7253.6343408E-919.0328121
GTCTACC3657.9909415E-418.9024521