FastQCFastQC Report
Mon 18 Jul 2016
MG317748_S6_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG317748_S6_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482186
Sequences flagged as poor quality0
Sequence length32-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGCC604.942605E-576.99644
AGGGCCT1006.1757886E-446.197845
CAGAGCG1450.003832431531.860589
ATAGGAC1853.346314E-431.2147623
GTGTATG3502.2277163E-829.7016931
TTAGGGC2607.808622E-526.6526013
TAGACTG2607.808622E-526.6526015
TATAGGA3906.377377E-826.6526012
AGGTCTC1750.00960417426.3987667
TCCTATA3108.603232E-626.0794282
GTATATA6300.025.668131
CTTAAGC2701.0093222E-425.665472
CTAGTAC2701.0093222E-425.665473
TTAAGCC2350.00135484924.573323
TAAGCCT2851.4570769E-424.3146554
TACACTG4301.6408376E-724.173295
ATGTAGG2400.001530679624.0638711
TACACGC2400.001531604624.0613757
CCTTAGG2951.8396745E-423.4928651
GTATTAT8600.022.8326991