FastQCFastQC Report
Mon 18 Jul 2016
MG317748_S6_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG317748_S6_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482186
Sequences flagged as poor quality0
Sequence length32-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG2901.8189894E-1243.80461
CGGCGCT1150.001229346640.1686489
AGGGTGT1551.1840777E-437.253186
TAGGGGT1350.002701504634.2177354
ACGGCGC1550.00531312329.8025468
CCTTAGG2753.3913366E-629.3961451
TACTGGG3152.9425428E-729.3294892
TATAGAC3602.9349394E-828.8712143
GTCTTAT5553.6379788E-1227.0505081
TAGAGGC1750.00960812426.3965383
ACCGAGC2209.226739E-426.2465578
ACAGCGT2851.4579111E-424.3126038
TATATAC6651.8189894E-1224.3126013
ACAGTAC3401.7615523E-523.7762958
CCTACAG2951.8420217E-423.4884472
CTGGACC2500.001943227123.0969724
TATACCG4552.833567E-722.8431595
TACCGAG4052.634375E-622.8118237
ACAGTGG4153.2557036E-622.2621426
TAGACAC2600.002440703622.2086265