FastQCFastQC Report
Mon 18 Jul 2016
MG038849_S11_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG038849_S11_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences895192
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGCG2158.306464E-426.7262299
ATAGGGG3057.890681E-626.3757213
GTATATA11000.026.1188131
TAGGGTC2250.00108306325.5383955
GTCTAGG3801.5819751E-624.194271
GTGCCTA3351.6331767E-524.0137141
CTATAAG5852.5102054E-1023.5739041
CCTATAC7105.456968E-1222.6608283
GTACCCT2600.002512515322.1005364
TAGGGGC3152.9709327E-421.8900554
TGGACCC2650.002806283521.6835445
GTATAGG5355.948641E-821.4808921
AGGGGCG2700.003127711821.2819965
GGACTCT3253.6695277E-421.2165136
CCTAGAG4355.1158186E-621.1352233
GTGTAAG6601.1368684E-920.895051
GTATAAG9400.020.7839071
CCTAGAC3905.2819047E-520.6271653
ACCTAGG2850.004278362320.161891
GTATAGA9200.019.986571