FastQCFastQC Report
Mon 18 Jul 2016
MG038849_S11_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG038849_S11_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences895192
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG8100.032.63085
GTAGGGC1750.00984610426.2667794
TACCGTG3851.7733055E-623.8788917
CTAGAGT4052.7539427E-622.6996863
CACTGAG3152.9719138E-421.8889857
GTGCACA2650.002807079621.6824844
ACAGTAC5957.850758E-921.245198
GTCCTGG4355.117983E-621.134191
ACCGTGC4456.2308627E-620.6592668
CTATAGT4557.5513253E-620.2052171
CTAGGAC4557.5513253E-620.2052173
GTCTAGG5159.800679E-720.0826111
CTTAGAC4608.2994175E-619.9855943
ACTGTGC4659.111927E-619.7706978
TAGGCTC4659.111927E-619.7706975
GTATAGG6401.8166247E-819.7513891
GTATAAT15600.019.152861
TAGGACC4209.341106E-519.152864
CTAGGCT3150.007630022718.2408224
ACACTGA10850.018.0054556