FastQCFastQC Report
Mon 18 Jul 2016
MG038315_S9_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG038315_S9_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences720038
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGGG250.0086529935135.65002235
TAGGGTC1601.4675377E-435.9216464
CTATACA7200.025.5442814
TAGACTG4151.2203236E-724.9287595
TAGTACT4002.4651508E-622.9898554
GTATTAG15150.022.7622321
GTATAGA8001.8189894E-1221.552991
CCTATAC4855.488546E-721.3307933
GTCCTAG3253.6628576E-421.2214051
GCACAGT3304.0605868E-420.8998666
CTTACCG3854.7720954E-520.8998669
CTAGTAC4608.275714E-619.9911793
GTAGACT5301.2864875E-619.519694
GTATATA8855.456968E-1219.4829271
CCCTTAC3556.6429906E-419.4280471
GTATTAT14350.019.2249661
TCCTATA5451.6806698E-618.9824492
GTATAGG4851.3067654E-518.9607051
CCCACAC3100.00694485518.5402073
CTATATA8107.203198E-1018.4486482