FastQCFastQC Report
Mon 18 Jul 2016
MG038315_S9_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG038315_S9_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences720038
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGC1406.690998E-541.0469866
TAGAGAG2801.09390385E-732.837595
CTAGGAC3052.3162283E-730.1459853
CTATAGG2701.0441062E-425.5403482
GTGTGGG2500.00199921122.9863131
ACCGTGC3152.9686178E-421.8917268
GTCCTAG3203.302055E-421.5496651
TATACTG8151.8189894E-1221.1530485
GTATATG8950.020.5464251
CTCTGTG2800.00385917720.5234937
GGAGTGC2850.0042756720.1634338
AACCGTG4608.286739E-619.9880987
GTCTACG4057.054903E-519.8647161
ATACCGT8703.6379788E-1219.8157866
TTAGACT4659.0980175E-619.7731724
GTATAAG8755.456968E-1219.7025531
TAAGGGC3607.306141E-419.155264
TAGGACT5451.6831491E-618.9795254
GTATATA8506.730261E-1118.9299051
CCTAAGC3050.00632833118.841242