FastQCFastQC Report
Mon 18 Jul 2016
MG019920_S4_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG019920_S4_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences666874
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCTAG2050.003335902232.775818235
GGGGTAT2804.1890326E-628.6121046
ATAGTGC3805.421316E-827.1062038
ATAGGGG2158.507265E-426.615913
GACCGTG3058.144132E-626.2668517
CGTATAC5902.9467628E-1023.2776433
GTATTAG13300.022.3733751
GTGTAAG5255.0853487E-821.7996981
TAGACCG2700.003202795721.194155
TATAGGG3304.1815976E-420.8088022
TATAGTG6101.0950316E-820.638245
CTGGTCC3905.4501397E-520.5420239
ACCGTGT3354.6281313E-420.4982248
GTATAGG5058.437637E-720.3967481
GTGTAGA7952.7284841E-1120.1544381
TAGACAG5701.2122655E-720.0786695
GTCTTAG5151.0184613E-620.0006941
CTAGGAC3506.2174385E-419.6197283
ATTATAC11850.019.3161893
GTAGAGT4158.787827E-519.3045524