FastQCFastQC Report
Mon 18 Jul 2016
MG019920_S4_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG019920_S4_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences666874
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTATA2708.262032E-833.902931
GGGTAGG1450.00401256131.56481
CCCTAGC1500.004736549730.5126383
TATACTG8300.026.1930775
CTAGGCT2209.7420777E-426.0050894
TATATAC9700.023.5922473
GTCCTAG3452.1199303E-523.2161391
TACACTG5604.094545E-922.4758285
ACTGAGC4254.3406144E-621.5383348
TAGGACT5406.866685E-821.1893334
CCTATAC4507.1196628E-620.341763
CTATACA7402.2919266E-1020.101234
AGACCCT3455.6517945E-419.8995486
TAGAGTG2900.004851010619.7285
TCCTATA6452.0849257E-819.5138972
CTAGAGT2950.005354982319.3936253
GTATAGG4158.803184E-519.3001611
ATGTGTG4209.650955E-519.07047
CTGCGCT3000.00590113219.0703989
TTAATGT7253.8580765E-918.9388794