FastQCFastQC Report
Mon 18 Jul 2016
MG017536_S5_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG017536_S5_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences825848
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGAC3653.527748E-828.3350013
CTAGTAC5302.064553E-923.8501133
GTGTAAG4852.1047526E-823.6936441
TATACTG11050.022.8788415
TAGTGTG4052.7541355E-622.6990975
TACACGG3552.5586305E-522.6591345
GTATAGA8453.6379788E-1220.3989661
CTGTGCG2950.005224892419.4769789
GTATTAG16950.018.9828721
TATACAG10300.018.9664175
GGATAGG3658.0258533E-418.8900011
GGTTAGG3658.0258533E-418.8900011
TAACACT7354.3419277E-918.7614974
GTATATA9901.8189894E-1218.571991
GTCCCTA3100.00695795118.5345421
ATAGCAC4401.3346678E-418.2818013
CCTACGC3150.00763067918.2403453
TGTAGAC6353.6205347E-718.096723
ATAGGAC4451.4553261E-418.0763873
ACTGTGC4451.4553261E-418.0763878