FastQCFastQC Report
Mon 18 Jul 2016
MG017536_S5_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG017536_S5_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences825848
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGTG1400.003308823832.8335465
AGGGTGC1650.007389370427.8587676
TACCCCG1700.00854798427.0393895
CTAGGGT1700.00854798427.0393894
TGGGTCG2608.086222E-526.5194025
CCGTCCC2300.001231408224.9820489
TACACCG6950.024.8023195
TAGACTG3801.582368E-624.1931425
CTTATAC10100.023.8937193
TATACTG9900.023.215645
ACAGTGC4002.4718265E-622.9834848
GCCTTAT5503.1668606E-922.9834821
TAGGGCT2500.002000890422.9834824
AGGGGGC2650.002806861721.6825318
TAGGGGT2800.003862403320.5209674
GTATAGG5159.798587E-720.0826551
GTAGGCT3455.491288E-419.985643
TATACCG4608.297984E-619.9856385
TCCTAGA7502.582965E-1019.9190182
GGAGTGT4057.063192E-519.8622686