FastQCFastQC Report
Mon 18 Jul 2016
MG016178_S2_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG016178_S2_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences871173
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTGG2856.2938343E-627.154581
GTATAGG6550.027.0065161
CCAGGAC3301.9616054E-523.4516813
GAGCGTC2500.002505177422.1115867
CAGGTCG2550.002810370221.6780269
ACAGTGC4604.8006405E-721.63098
GTGTAGC9700.020.5159051
ACCGCGT3254.7652627E-420.4106968
GTACTGC6151.7920684E-819.7745916
GGACTCC2850.00535227919.3961286
CTAGCCC2850.00535227919.3961284
CCTCGTA2900.005918105619.0617122
GTATATA10450.019.0434721
TAGACTG4901.9972109E-518.0502745
GTCCTAA6755.2099494E-818.016851
CATGCGC3100.00869493217.8319248
AGTGTCC3100.00869493217.8319248
TACACCC3750.001247441317.689274
GTGTACG3150.00953422317.5488781
CCTTACA5102.8167822E-517.342422