FastQCFastQC Report
Mon 18 Jul 2016
MG016178_S2_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG016178_S2_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences871173
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGTG1854.331591E-429.873269
TAGGGTC2006.835383E-427.6327675
GTAGGGT2109.0895704E-426.316924
GTCTATA7150.026.2801131
GTCCTGG3009.391306E-625.7905831
GTATACA10400.022.3187711
TAGTCCC3153.8655536E-421.0535344
CTATACT8450.020.9289594
CTAGGAC4858.003117E-720.510923
ACAGTGC4458.722676E-619.8707528
TGGACCG3907.127956E-519.8389075
GGACCGT3406.468962E-419.5054826
ACCGTCG2850.005359804319.3914158
TACACAG8004.9112714E-1119.3429365
ACACAGT5751.9199615E-719.2227946
CCATGCG4059.525177E-519.1041369
GCTAGAC4701.397939E-518.8137973
TACGGTG4751.5314043E-518.6157595
GGGAGGA4751.5314043E-518.6157597
CCTACAC6003.003588E-718.4218443