FastQCFastQC Report
Mon 18 Jul 2016
MG016017_S1_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG016017_S1_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1178907
Sequences flagged as poor quality0
Sequence length32-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGTG1653.5801E-430.8618249
GTATAGG6250.026.0720671
GTATAGA9850.025.8487341
TTAGGCC2300.00248707922.1400054
TACACTG9950.021.4947175
CTGTGTG2903.8448683E-421.0711779
GTATTAG19650.019.6950261
CTATACT11050.019.3549734
TAGGTCG2650.005650210719.215855
ACCGTGC4301.3156656E-518.9477238
GTACTAG6504.2327883E-918.8019711
CTAGGAC3901.3364594E-418.2796963
TAGTGCG4001.6223574E-417.8227049
GTATATA14400.017.6812531
TATACAG15150.017.4781825
TTAGGGT5905.9462946E-717.2616984
CACAGTG4152.1497767E-417.1785097
TATAGAG9555.456968E-1217.062872
TAGGGTA4202.355685E-416.9740035
TATACGG7201.50103E-816.9740035