FastQCFastQC Report
Mon 18 Jul 2016
MG016017_S1_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG016017_S1_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1178907
Sequences flagged as poor quality0
Sequence length32-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCCGT855.1673426E-447.9050066
CCGCCCG1602.996961E-431.8119189
CTGTGTG2555.037944E-627.9445889
TACCCCC2050.00127669324.8288145
TAACGGG4900.002821323721.661896235
ACTGAGC5801.031367E-921.0616848
CCTGGAC4751.4439429E-619.2880693
TATAGGG4301.3207857E-518.939193
TAGACTG6053.8309736E-818.508755
TACACCG10100.018.1422425
TAGGACC3450.001240840617.7040234
CCTGTGG3500.001366475417.4511098
TATACCG7601.5606929E-917.4128385
GTATACG11750.017.3273411
GTGTAGA11250.017.1925741
TAGGACT7201.5093974E-816.9663564
TATACAG15050.016.9099885
ACACTGG6651.1287011E-716.8387896
CTAGTAC6108.4676867E-716.688223
CTAGGAC4904.0592837E-516.6201043