FastQCFastQC Report
Mon 18 Jul 2016
MG015602_S3_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG015602_S3_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences846545
Sequences flagged as poor quality0
Sequence length32-241
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATG7400.024.686071
CCGTGCG2400.001638753923.7860579
GTATATA6504.1836756E-1122.8346121
GTGTACG3354.7053778E-420.4489081
GTGCGCA2800.00401061620.3880489
TAAGCCC4006.748925E-519.9802864
GTATAAG7704.0199666E-1019.2759721
GTATTAT15550.019.090031
TATACCG5401.6425511E-619.0288455
TCCGTGC3709.185429E-418.5145538
GTATACG5051.9626501E-518.0868231
ACAGTGC3800.001098654518.0273278
GTATAAC6454.5652814E-717.7012521
CTTACAC5252.740563E-517.39783
ACTAGGC4000.001549438217.125968
GTATAGG5403.4901146E-516.9145281
ATACCGT8852.568413E-916.7711836
TAAGAGT5504.0846397E-516.606994
CCTGGAT6206.1370447E-616.573513
TAGACAC4150.00198210116.5069485