FastQCFastQC Report
Mon 18 Jul 2016
MG015602_S3_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG015602_S3_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences846545
Sequences flagged as poor quality0
Sequence length32-241
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG10300.027.6973135
ACCGTGC2701.0962918E-425.3584298
CTAAGGG4251.6492777E-724.1650923
GTATAGG5604.2291504E-922.4150411
GGGTAGT3102.79669E-422.0863761
ATAGGAC4653.927762E-722.0863743
CCGTGCA3304.2665732E-420.7478059
ACGGCCT2800.004022869720.377318
ACTGTGC5151.0482308E-619.9420668
TAGGACA6552.568595E-819.1640034
GTATTAT16100.019.1369531
GTATATA5501.9676736E-618.6730271
CTTAGAC5501.9676736E-618.6730273
GTGTAAG5001.809771E-518.258071
ACAGCGC5102.1459306E-517.900078
CCTGGAC3200.00869973717.8301473
CTTATAC7851.0699296E-817.4440163
TATAGGG3950.001429294617.333612
GGACAGT5403.505919E-516.9056196
GTTATAC6807.982726E-716.7813153