FastQCFastQC Report
Mon 18 Jul 2016
BU845243_S16_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBU845243_S16_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences859332
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGC1350.002738333834.126388
GCCCTAC1750.00973553826.3275971
AGGGTGG2350.001377669624.5056448
CTCTGGG2851.4859166E-424.247692
ACCGTGT4751.6467311E-824.247698
ACTAGAC6453.274181E-1123.2138733
GTGAGCG2550.002215858522.5836359
CACCGTG4103.0039137E-622.4734697
CTGTGTG3602.8009497E-522.3954379
AGGGTGT2700.003087857521.3289876
TAGACCG3804.2508756E-521.2167285
GTGTATA7801.8189894E-1120.6739141
GTCTATA7301.7462298E-1020.5120831
CTAGACG4751.0737593E-519.3981534
TAGAGCT4751.0737593E-519.3981534
CTAGACA6102.318211E-718.8813974
CTGTGCA4451.4302172E-418.1176579
CCGTGTA3850.001130995817.9495899
TATATAG7758.139978E-917.8337865
CTATACT9101.72804E-1017.7194674