FastQCFastQC Report
Mon 18 Jul 2016
BU845243_S16_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBU845243_S16_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences859332
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCCG1253.3832315E-546.0761035
AGGCCGA1600.00628543428.7975676
GCCCTGA1600.00628543428.7975671
ATAGGAC4103.430614E-928.0951883
GTACTAG5601.4188117E-1024.6836281
CTATACT9150.023.919294
ACTATAC6751.8189894E-1223.8913143
TATACAG12700.023.5822585
ACCGCTC5103.4968252E-822.5863278
AGGGTGT2600.00247874322.1519746
CCTATAC4905.939455E-721.1573963
CTTATAC9500.020.6129933
CCCTATA3505.954625E-419.74692
GTGTAAG6451.9326762E-819.6448521
CCGTACG2950.00515307119.5237739
GTATAGG6702.9900548E-818.9118351
ACTAGGC3050.00624766918.883653
CCCGTAC3050.00624766918.883658
CAGTGTG6102.3153007E-718.883659
GTCCTAG4901.4025383E-518.8065741