FastQCFastQC Report
Mon 18 Jul 2016
BU814330_S22_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBU814330_S22_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012067
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTG6403.45608E-1123.1439465
GTATAGG6551.1514203E-920.87441
GACCGTC5008.0125756E-720.5090967
TATACCG7352.2191671E-1020.1525545
TGGACCG4751.17895925E-519.1897985
ACCCACC3050.00665493618.6785968
TAGAGTG5001.8342063E-518.2303095
TATAGGA10051.8189894E-1218.139612
GCCGTGC3150.00801601118.0856258
AGAGTGT5152.3651633E-517.699336
CTATACC5903.904246E-617.380594
CTTACAC7901.1799784E-817.3072553
GTCTAGA6656.425762E-717.1337491
GTGTATA8201.8695573E-816.6740631
TCTATAC9604.3473847E-1016.6161673
ATAGAGT7601.4308534E-716.4912343
GTATAAG8352.3377652E-816.3745291
ATATACC9053.5524863E-916.366994
AGGACCG4903.2428434E-416.2770615
GTACTAG7101.2737273E-616.0478081