FastQCFastQC Report
Mon 18 Jul 2016
BU814330_S22_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBU814330_S22_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012067
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGGC1700.00891499126.8078783
ACCGTGC3907.293238E-826.292348
AGGCTAG3550.00130544724.732103235
TACACCC4701.652188E-824.2411654
GGTCTAT6701.8189894E-1223.8069951
TATACAG14600.023.4109885
TAGGGCA3102.8271473E-422.0516414
ACAGGGC3354.7734033E-420.4059968
ACACCCC3455.8200693E-419.8145185
GTCCTAG5401.6765716E-618.9889131
ACTGTGC6052.3840221E-718.831988
CCACACT4851.4116014E-518.793154
GTCTAAT9151.0913936E-1118.677621
CCCTACA5552.1789056E-618.4756982
CCTAGAC3750.001019660518.2293573
TGTGTGC4451.5544628E-417.922128
CTCTAGG5102.1758558E-517.8719161
ACTGCCC3900.001326513217.5282278
CACCGTA7157.1373506E-817.5282277
CTAGGCT3900.001326513217.5282274