FastQCFastQC Report
Mon 18 Jul 2016
BU795895_S18_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBU795895_S18_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences710147
Sequences flagged as poor quality0
Sequence length32-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTGTG2350.001430735124.3464497
CCTACAC4052.8564136E-622.6031253
TAGACCG2650.002876805821.5902485
ACTGTGC2650.002876805821.5902488
CTAGGAC2750.003566100720.8051473
CTATACT7201.5825208E-1020.6606674
CACCGGT2800.003958465520.4336287
GTATAGA7452.5102054E-1019.9673581
CCTATAG3455.6503207E-419.9005781
TCTAGAC4108.01831E-519.5365413
GTGTATA7653.5652192E-1019.4453331
GTCTATG5901.7471211E-719.394631
GTATAAG8855.456968E-1219.394631
CTATAGG3556.849092E-419.3399962
CCTGCAC4801.24275775E-519.0713863
CTGTGCT4251.0565419E-418.8470179
TATAGGC3050.006490617518.758743
GGGTATA4301.15569754E-418.6278651
TATACGA10450.018.6151335
TATAGAG8158.3127816E-1018.2523692