FastQCFastQC Report
Mon 18 Jul 2016
BU795895_S18_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBU795895_S18_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences710147
Sequences flagged as poor quality0
Sequence length32-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTG2303.602635E-529.8594347
TATACTG9650.029.6531525
CCCTACG1550.00555121529.5383662
CACACAG1954.8038873E-429.3490185
CATACAC6001.2732926E-1124.799923
TACACCG6551.8189894E-1224.4649815
TAGGTTG4452.4953988E-723.1494485
GTATAGA8000.022.8922351
GTATATG9100.022.640671
TATAGTG7105.456968E-1222.5698075
TAGACGT4603.4352888E-722.3945755
CAGAGAG3153.0520244E-421.8021265
TAGAGAG3703.629936E-521.6548165
TAGACAG6509.877112E-1021.1312925
GTATAAG8151.8189894E-1221.0664711
GTATACC4906.310129E-721.023484
TAGACTG3905.446103E-520.5443125
GTGCAGG5058.4301973E-720.399021
ACCGTGT4507.099903E-620.3486528
ACTAGAC6201.3189492E-820.3076273