FastQCFastQC Report
Mon 18 Jul 2016
BU768105_S14_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBU768105_S14_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences853217
Sequences flagged as poor quality0
Sequence length32-241
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAAGG2351.0273125E-634.2622343
GTGTGCA3102.653196E-729.6834079
TGTGTGC3558.676143E-725.9207238
GTTAGAC5352.2755557E-923.6496323
GTATAGG5355.893162E-821.4996641
TAGACAG8600.021.3996685
TCCTAAG6458.440111E-1021.3996682
GTATATA8600.021.3996681
GTCTAGG3354.4757547E-420.6011711
CAGCGCA4057.013744E-519.8805549
GTCTAGC4659.038809E-619.7889391
AGACCGT7052.557499E-919.5784176
TCTAGAC5301.2791534E-619.5322443
GTGTTAG6552.3448592E-819.3168751
GTCCTAA6552.3448592E-819.3168751
GTTATAG6552.3448592E-819.3168751
TACCCTC3000.005726550219.1705345
TAGGACC3607.268575E-419.1705344
ACACAGT5451.6710983E-618.9946586
TAGAGTG4251.01563935E-418.9455