FastQCFastQC Report
Mon 18 Jul 2016
BU768105_S14_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBU768105_S14_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences853217
Sequences flagged as poor quality0
Sequence length32-241
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG6100.032.0583575
GTGTGAG1904.007946E-430.2718261
CCTAGAC3203.5006997E-728.7582343
TAAGACC4255.1077222E-927.0665744
CCTTAGG2751.17590476E-425.0980951
CTAGACC5101.3060344E-924.8110264
GTACTAC3251.2818367E-524.7763251
CCCTATA3301.442798E-524.4009272
GTGTGCT3301.442798E-524.4009279
TACACCG8750.022.3492575
CCTATAC5305.331276E-821.7043273
TCTACAC10100.021.639863
GTCTTAG7802.0008883E-1120.6469361
GTCTAGG3354.4732E-420.6029151
AGTGTGC2800.003840093720.5415958
TTAAGGT4506.8030786E-620.45034
TAGACTG3506.0096424E-419.7199335
GTGTTAG6052.1539927E-719.013711
TATACGG7455.0695235E-918.5287955
GCATGGG4600.00725152718.401129235