FastQCFastQC Report
Mon 18 Jul 2016
BU767578_S15_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBU767578_S15_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences998033
Sequences flagged as poor quality0
Sequence length32-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGGCA3851.8273404E-623.7971639
CCGTGGC2500.002041497322.904779
GTAGTAC4855.692327E-721.2518483
GTATAGA9850.020.9282171
GTATACT9850.020.9282174
GTATACA9900.020.822521
GTCTTAG7552.9649527E-1019.7193371
TATACTG12450.019.3172765
GTCTAGA6803.788773E-818.5259151
TAGATCG6803.788773E-818.5259155
TATATAC13650.018.4580573
GTATAGT8751.07320375E-1018.3238161
GTATAAG11250.018.3238161
ATAAGAC12050.018.0577013
GTGTAGG7005.2796167E-817.9966051
GTGTAAG7657.4414856E-917.9645251
GTATTAG20100.017.6628821
CTTAGAC5903.7183036E-617.469743
ATTATAC17200.017.3117453
CAGTGCA5302.8965023E-517.286629