FastQCFastQC Report
Mon 18 Jul 2016
BU767578_S15_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBU767578_S15_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences998033
Sequences flagged as poor quality0
Sequence length32-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGCC1150.001282225639.8302279
GTCCTAG4900.037.3916441
CCTAGGC1550.005540267629.5514583
TATACCG6351.8189894E-1225.2467175
TCCTAGA8400.021.811792
CATATAC12300.021.4127943
CTAGGCT3304.1679753E-420.8203454
CAGACCC2750.003552038520.8203459
AGGACCG3354.6130622E-420.5095945
ACCGTGC4006.598375E-520.0395818
TACTCCG4057.259706E-519.792185
ACCGTGT5901.7354978E-719.4087968
TAGACCG4901.4761659E-518.6958225
CCTATAC6803.7933205E-818.5239833
GTACTAC4951.6110851E-518.5069731
TAGACTA7556.330083E-918.2005675
AGTGTGC3800.00107720618.0808288
AGACCGT8259.713403E-1018.04436
GGTATAG6353.758214E-718.0333711
GTACTGT7106.21676E-817.741286