FastQCFastQC Report
Mon 18 Jul 2016
BI123566_S17_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBI123566_S17_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences854961
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG5351.8189894E-1227.9292011
TAGACAG6250.025.7464245
GTCTAGG3751.4077905E-624.5204071
TATACCG6101.6370905E-1124.4952815
TAGGACA6255.7480065E-1022.0683634
TAGGACC3703.5161163E-521.7452934
TACACTG6357.003109E-1021.7208335
GTATAGC8251.8189894E-1220.8980731
GTATAGA8301.8189894E-1220.772181
TAGATAG10150.020.3833435
ACTGTGC3955.8192272E-520.3690098
CCTGCAC6251.3798854E-820.2293343
CATAGAG3455.484431E-419.989462
GTATATA11050.019.7633361
GTATAGT6401.8122591E-819.755211
CTAGGAC4107.750749E-519.62383
ATAGGAC4709.976036E-619.5641543
TATAGGG3556.648227E-419.4263782
TTAAGAC6001.9908657E-719.1565673
TACCCTG3050.00632678618.8425245