FastQCFastQC Report
Mon 18 Jul 2016
BI123566_S17_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBI123566_S17_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences854961
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGGCC1250.001896265136.7805144
ACCGGGC1651.7597548E-434.8300328
CCGGGCA1600.00635278828.734789
CAGTGCC3906.695336E-826.524419
TAGGGGT2751.182442E-425.0776254
GTAGACC3301.45195045E-524.3810234
TACACCG9150.022.6109735
GTGTACG3653.1657277E-522.0431181
TAGAGCT4855.4956945E-721.3289094
GTATAGG5406.5527274E-821.285021
GTCTAGG3804.3189888E-521.1729951
GCTACAC8553.6379788E-1220.1647553
TATACTG9150.020.0986425
TATAGTG6901.9754225E-919.989415
TACGTCG2900.004727286819.8170875
GTATAGA8153.6379788E-1119.7441431
GTGTAGG7002.3628672E-919.7038481
ACACTGC4107.7509045E-519.623756
TGTAGGG4709.976258E-619.5641042
TATACAG12200.018.8424785