FastQCFastQC Report
Mon 18 Jul 2016
BB420982_S12_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBB420982_S12_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences604030
Sequences flagged as poor quality0
Sequence length32-241
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG1909.556061E-636.2508431
ATAGGAC3304.661997E-727.828933
GTATAGG3552.5778596E-522.6355021
GTAGGGC2550.002258301222.5086923
TCTACAC7257.2759576E-1222.1671833
TCCTAAG4153.431991E-622.1290262
TAGGCTG3653.1946733E-522.0153525
ATCCTAC3455.528724E-419.9642311
GTGTACA5201.085129E-619.8682481
TATTATA8304.9112714E-1119.3628982
TAGGACA4158.584789E-519.3628984
TAGGGAG3607.363219E-419.132395
TATATAG7404.776666E-918.6152975
CTATTAC6803.680725E-818.569671
GTCTATG4351.2318724E-418.472651
GTCCTAA5051.8716644E-518.185241
CTACACT3800.001058884818.1254224
GAGCCAC3200.00840366717.9366139
CAGGACA5403.328829E-517.0065674
GTCTTAT7451.04495484E-716.94951