FastQCFastQC Report
Mon 18 Jul 2016
BB420982_S12_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBB420982_S12_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences604030
Sequences flagged as poor quality0
Sequence length32-241
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGTGT2308.803654E-734.9423456
TAGGCTG3956.730261E-1131.972565
CTACTCT1450.003946300631.6718799
GTGTATG6500.028.261061
ATAGGAC2951.9173732E-423.35133
CACCTCG2600.002525867422.0789537
GTCTATA4203.8030248E-621.8686771
CCTAATA7901.8189894E-1221.7994732
TAGGACC2650.00282118821.6623694
TTATACT5356.002301E-821.4599174
CACCGCG2700.003144312921.2612157
GTGTATA4405.690883E-620.8746451
AGGGTAT4456.2755335E-620.64018
ACTACTC3955.8660233E-520.3461748
TAGACAG3405.005842E-420.2606875
ACCGAGC2850.004301031620.1422028
CAGGGGA2850.004301031620.1422024
CTAATAC9250.019.8591213
TCTAGGC2950.005250610419.4594153
CATAGGA2950.005250610419.4594152