FastQCFastQC Report
Mon 18 Jul 2016
BB417939_S19_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBB417939_S19_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1016770
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTG5750.027.9732465
GTATATA11350.023.2816961
ACTGTGC5452.8594513E-923.188838
TCTAGAC5603.912646E-922.56773
TATACTG12350.022.3268245
CAGGGCA4153.4110453E-622.1474919
GTCTAGC6255.7843863E-1022.0589031
CCTTAGG3905.2943862E-520.6213021
ACAGGGC3354.511669E-420.5773338
TACACCG10050.020.5773335
GTCTATA8053.092282E-1119.9808861
CTTAGGG4107.7781915E-519.6153853
GACCGTG4158.537286E-519.3790557
CCTACAC8405.820766E-1119.1483523
CCCTTAG3607.3267333E-419.1483521
GCAGGGC3000.005765730519.148358
GCGGAGG3658.038906E-418.8860458
CTTACAC8557.4578566E-1118.8124163
GTGTTAG7354.3564796E-918.757571
CACTGTG4301.1211554E-418.7030417