FastQCFastQC Report
Mon 18 Jul 2016
BB417939_S19_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBB417939_S19_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1016770
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG11000.024.0113725
AGGGAGC4351.9431536E-723.7593786
GGGAGCG3102.6800766E-422.2265157
CTACACT7901.8189894E-1221.8044934
TATAGGG4754.5377965E-721.7585892
TACACAG7451.0913936E-1121.5801075
CCCCTGT3203.321773E-421.5319391
CTATACT9100.021.4530664
TCTACAC11300.021.3413893
TACACTG10250.021.2868585
ACACTGT7902.3646862E-1120.350866
GTATATG12500.020.2113111
ACCGCCC5159.869218E-720.0686028
TATACCG6952.1864253E-919.827975
ATAAGGG9300.019.7569033
GGAGAGT5301.2996679E-619.5006226
GTATACT9450.019.44334
GCTACAC10700.019.3183733
TAGTACT7153.1104719E-919.2733424
ATAGGGC3000.005781152819.13953