FastQCFastQC Report
Mon 18 Jul 2016
BB416467_S21_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBB416467_S21_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences546176
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGG3456.648952E-726.7198891
CCTCTAT4755.6024874E-1026.684731
GTATAAG5751.9645086E-1024.04791
GCCTTAG2901.6656597E-423.8405911
GTGTTAG4351.8768333E-723.840591
CTATACT6453.274181E-1123.2245544
GTCTTAG4603.217956E-722.5449051
GTACTGT4102.9886323E-622.4838091
AGACTGA3653.102012E-522.0988126
GTGCTAC2800.00380057520.57671
CAGTGCA3455.388624E-420.0399179
GCTATAC6351.605622E-819.9610183
GCAGTGC2900.004656662219.8671598
GTAGGAC3505.937174E-419.753633
TAGACTG2950.00514057819.5304265
GAGTGAC3556.5322267E-419.475417
ACAGGAC4801.1693615E-519.204923
TCCTATA4301.09489614E-418.7582932
CCTTAGA3708.6288556E-418.6858672
TTAGACA7404.5547495E-918.6858674