FastQCFastQC Report
Mon 18 Jul 2016
BB416467_S21_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBB416467_S21_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences546176
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGG700.00885184349.3839847
TAGGACC2308.5544707E-735.0697824
AAGGGGG2252.9610039E-530.727815
GGACAGT4551.0355507E-825.3251196
GTATAGG3251.26392115E-524.8186171
TATAGTG4701.4621037E-824.5168695
AGTACCG3801.54388E-624.2587975
ACAGTGC2450.001750477823.516188
GTATAAT9000.023.0458561
CAGTACC4502.6034468E-723.0458564
TACACAG4552.896213E-722.7926065
CAGTGCA3152.91589E-421.9484359
GTATTAA8001.8189894E-1221.6054921
TTGGACT6958.913048E-1121.5536794
TAGAGCG2750.003423809320.950785
GTCTAAT5701.127446E-720.2156661
GCCATAG3455.3886964E-420.0398771
ACAGCGT3455.3886964E-420.0398778
CCTATAA4158.337147E-519.4362662
CCTACAC3607.177041E-419.2048823