FastQCFastQC Report
Mon 18 Jul 2016
BB412635_S20_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBB412635_S20_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences639028
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGAG2652.6170837E-630.3928625
TATACCG4251.5643309E-1029.7798985
GTATTAG11750.023.501351
GCACCGT4052.7226379E-622.7276426
TAGAGTG3102.6438455E-422.2694245
GTACTGG4804.920712E-721.5735051
TATACAG10300.021.2244195
GTCTATA4956.6151915E-720.9197621
GTGTAGG4506.785529E-620.4548781
GTAGTGT2900.004697660419.8377061
ACAGGGC2950.00518580619.5014728
TATAGGG2950.00518580619.5014722
ATTAGAC7804.292815E-1019.1764493
TGCACCG4251.01273174E-418.9508445
ATAGGGG3050.006287306518.8620823
ACAGTGG4301.1077999E-418.7304848
CCGTCTA4451.4407406E-418.0991219
CTCTATA5101.9976216E-518.0484222
TAGACCG3200.00829351817.977925
TATTAGG10900.017.9449332