FastQCFastQC Report
Mon 18 Jul 2016
BB412635_S20_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBB412635_S20_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences639028
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGC1853.4354316E-431.0770538
CAGGTCG1550.0054282729.6735749
GCCTATA3108.909521E-625.9643761
TACACCG6551.8189894E-1224.5769675
TATACAG8700.023.790025
TACACTG6003.43789E-1022.9970195
ACCGAGC3102.6553136E-422.255188
TTATACC7409.094947E-1221.7539374
CCGAGCA2700.003116929721.2935359
GTCTAGG2750.003466806820.906381
GTATAAT11950.020.2065851
CATGGGG2850.004263643620.1728234
CGTACAC2850.004263643620.1728233
GTATATA6851.7917046E-920.1433731
GCCTATG4659.058884E-619.7823811
TATACCG4751.0885697E-519.365915
CCTATAA5351.4028847E-619.3432871
GTCTAAC3000.005735984519.1641831
GTATAAG7354.303729E-918.7730751
TAGACTG3759.581281E-418.3976155