FastQCFastQC Report
Mon 18 Jul 2016
BB411906_S13_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBB411906_S13_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences926378
Sequences flagged as poor quality0
Sequence length32-241
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACT4059.543146E-825.5719784
GTATAAG8350.023.4320131
CTATAGA6705.638867E-1122.3313851
CCTAGAC5957.728886E-921.2741663
GTCGTAG4405.5765977E-620.9259171
ATCCTAG4006.345981E-520.1411931
CCTGGAC3556.5966696E-419.4491083
GTACACT4158.4320905E-519.4100534
CCCTAGA4751.0806898E-519.3840071
TCTAGAC5951.8011087E-719.340153
TACTGTG9201.0913936E-1118.7620497
GTATTAG14200.018.6417481
CCTAGCA3100.006903026718.5603072
AGGGTGT6253.0213232E-718.4118236
GTCCTAG3150.007563418218.2686561
ACCGCGC3150.00757048618.26578
ACCATCC14700.018.004768
GGGTAGG3200.00828220717.983211
TAGGGTC3200.00828994217.9802974
CTAGGGT3200.00828994217.9802974