FastQCFastQC Report
Mon 18 Jul 2016
BB411906_S13_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBB411906_S13_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences926378
Sequences flagged as poor quality0
Sequence length32-241
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGTG2303.473988E-530.0203695
CCGTACG2158.241428E-426.7623449
CCTAGAC6003.4015102E-1023.0156153
TAGACTG5553.4615368E-922.8082685
CTAGACT4153.362471E-622.1837254
TAGACAG5805.7552825E-921.8251535
GTGTGAG5701.1453267E-720.1891371
CTACCCT3708.7116996E-418.661314
TCCTAGA5602.1572087E-618.4946922
ACCGTGC5652.3484881E-618.3310228
TATACAG11950.018.2969325
CTACACT5702.554727E-618.1702234
CACTGTG6504.5581328E-717.704327
TAGGGCG3900.00124042917.704324
GTCCAAG8551.4624675E-917.4972521
TACCCTA3950.001351005317.4802155
CTAGACA5302.778539E-517.3702754
TAGTACA8051.3162207E-817.1544974
GTCTATG7409.424184E-817.1062011
TACACTG8802.1445885E-917.000175