FastQCFastQC Report
Mon 11 Jul 2016
Undetermined_S0_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameUndetermined_S0_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520647
Sequences flagged as poor quality0
Sequence length32-241
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC13780.2646706885855484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATAC3354.981921E-727.6170163
GTATATA4254.802132E-927.2187291
GTCTATG2851.4415545E-424.3535981
CTCTATA2500.001927713923.1292512
GTATACA3052.2828323E-422.7566411
GTATAGA3854.5417204E-521.0326521
GATCTAG2750.003347058821.0326521
CTATCCC2850.004123435420.2888164
TATGGAG3455.258641E-420.1123925
TATTAGG4607.847582E-620.1123922
TCTACAC3505.7940366E-419.8250713
GTCCAAT4107.370575E-519.7501741
GCTATAC2950.005034074719.601063
TATACTG4801.1333352E-519.2743765
CTACACA3100.006704326718.6526224
TACACCG3100.006704326718.6526225
GTATTAG5001.6073533E-518.5087361
TATTAAC3800.001007355318.2599352
GTATTAT5152.0730182E-517.9696451
GTTCTGT5202.2526152E-517.796861