FastQCFastQC Report
Mon 11 Jul 2016
Undetermined_S0_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameUndetermined_S0_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520647
Sequences flagged as poor quality0
Sequence length32-241
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAC1400.0032287332.995913
GTACCGT1903.914676E-430.3909726
GGACTAT1954.558018E-429.6117156
ATATACT5053.6379788E-1127.4421424
TACCCTG2158.068199E-426.8571385
CCCAGAC1750.00960847726.3967292
GTAGAGC2209.2273444E-426.2467483
TCCTATA2300.00119590725.1055852
TATACAG3851.6965005E-623.9970285
CTATACC2450.001727876123.5685084
ATATATG5502.9849616E-923.0971371
CTAGAAC2500.001943353123.0971363
TAGACGT2500.001943353123.0971364
CTAACCA2500.001943353123.0971364
CTAAGTA2600.00244086122.2087864
AGGAGCG3152.872142E-421.9972745
TTAGACT2700.003038626921.386243
GTCTATG2700.003038626921.386241
TCTATAC3253.547644E-421.3204363
GCAGTGC2800.003751762220.6224468