FastQCFastQC Report
Mon 11 Jul 2016
MG401829Y_S3_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG401829Y_S3_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences865776
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGGGG3005.59447E-428.593754235
TTATACT12150.026.372494
TATACTG11000.026.0085855
CTAGGCA3601.0247877E-625.4306164
CTATACT8700.023.6767794
TAGGCAG4304.800733E-621.2907495
GTCTACA7001.09139364E-1021.2564131
GTCGTCC3804.467056E-521.080649
ACAGAGC5701.2145028E-720.0768018
GTATAGA8204.3655746E-1119.5415421
ACTGTGC4108.0158134E-519.5381558
CTCTGTG3607.522523E-419.0729622
TCCTAGG3658.253609E-418.8116872
TCAGGGG3100.0071270118.4577033
AGAGGGG4700.00760153418.251333235
TCTATAC9552.0008883E-1117.974523
CCTATAC6454.456051E-717.7422893
GCTATAC7758.814823E-917.7193973
TATACAG11850.017.3829525
TTTATAC19200.017.284873