FastQCFastQC Report
Mon 11 Jul 2016
MG401829Y_S3_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG401829Y_S3_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences865776
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG2056.443133E-427.913495
ACAGGCC1700.00872156126.9283088
ACCGTCC3457.065446E-726.5380428
ACCGTGC3559.0660797E-725.790498
CTATACT8550.025.4322914
GTCTAGG3601.0242002E-625.432291
TTAGGGG3851.8378105E-623.7808424
AGCGTCC3452.1175956E-523.2207878
GTCCTAG3952.2968088E-623.1787971
GACCGTG3502.3667628E-522.8890617
GAGAGGG2500.002049437522.8890617
CTAGGGG2550.002299410822.4402564
ACTATAC7359.094947E-1221.7991053
AGGGGCG2650.002874832821.5934526
GCACGGC2700.003204062321.1935757
TATACTC8201.8189894E-1220.9351185
CCCTACA4405.8548594E-620.8082372
GTGTGGG3506.2206323E-419.6191961
GTATAGA9500.019.2749981
ACCGCTC4209.6403666E-519.0742178